期刊
NUTRITION RESEARCH
卷 34, 期 11, 页码 990-999出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.nutres.2014.10.001
关键词
Aging; BALB/c mice; Broccoli; Inflammation; LPS; Sulforaphane
资金
- National Institutes of Health [AG16710]
- US Department of Agriculture/National Institute of Food and Agriculture [2010-65200-20398]
Aging is associated with oxidative stress and heightened inflammatory response to infection. Dietary interventions to reduce these changes are therefore desirable. Broccoli contains glucoraphanin, which is converted to sulforaphane (SFN) by plant myrosinase during cooking preparation or digestion. Sulforaphane increases antioxidant enzymes including NAD(P)H quinone oxidoreductase and heme oxygenase I and inhibits inflammatory cytokines. We hypothesized that dietary broccoli would support an antioxidant response in brain and periphery of aged mice and inhibit lipopolysaccharide (LPS)-induced inflammation and sickness. Young adult and aged mice were fed control or 10% broccoli diet for 28 days before an intraperitoneal LPS injection. Social interactions were assessed 2, 4, 8, and 24 hours after LPS, and mRNA was quantified in liver and brain at 24 hours. Dietary broccoli did not ameliorate LPS-induced decrease in social interactions in young or aged mice. Interleukin-1 beta (IL-1 beta) expression was unaffected by broccoli consumption but was induced by LPS in brain and liver of adult and aged mice. In addition, IL-1 beta was elevated in brain of aged mice without LPS. Broccoli consumption decreased age-elevated cytochrome b-245 beta, an oxidative stress marker, and reduced glial activation markers in aged mice. Collectively, these data suggest that 10% broccoli diet provides a modest reduction in age-related oxidative stress and glial reactivity, but is insufficient to inhibit LPS-induced inflammation. Thus, it is likely that SFN would need to be provided in supplement form to control the inflammatory response to LPS. (C) 2014 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-SA license.
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