期刊
NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL
卷 66, 期 3, 页码 474-482出版社
ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD
DOI: 10.1080/01635581.2014.884236
关键词
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资金
- Association pour la Recherche sur les Tumeurs de la Prostate
- La Ligue Contre Le Cancer (Comites de la region Auvergne)
Major phytoestrogens genistein and daidzein have been reported to have the ability to reverse DNA methylation in cancer cell lines. The mechanism by which genistein and daidzein have an inhibiting action on DNA methylation is not well understood. The aim of this study was to investigate the effects of soy phytoestrogens and the natural estrogen 17 beta-estradiol (E-2) to determine whether one of the estrogen receptors is mobilized for the action of these compounds on DNA methylation. We also made a comparative study with a DNA methylation inhibitor (5-azacytidine) and a DNA methylation activator (budesonide). Three prostate cell lines, PC-3, DU-145, and LNCaP, were treated with 40 mu M genistein, 110 mu M daidzein, 2 mu M budesonide, 2 mu M 5-azacytidine, and 10 mu M E-2. In these 3 human prostate cancer cell lines, we performed methylation quantification using methyl-profiler-DNA-methylation analysis. Soy phytoestrogens and E-2 induced a demethylation of all the promoter regions studied except for those that were unmethylated in control cells. Our results showed that E-2 induces, like soy phytoestrogen, a decrease in DNA methylation in prostate cancer cell lines. This action may be mediated through ER beta.
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