期刊
NUTRITION
卷 25, 期 1, 页码 40-44出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.nut.2008.07.006
关键词
Histone H3 acetylation; Carbohydrate/fat ratio; Chromatin; Na+/glucose cotransporter-1; Small intestine
资金
- Ministry of Education, Science, Sports and Culture of Japan [18590220, 18790171]
Objective: We examined in this study whether histone H3 acetylations on jejunal NA(+)/glucose transport-1 (SGLT1) gene are associated with induction of its gene by feeding mice a high carbohydrate/fat ratio diet. Methods: The chromatin immunoprecipitation (ChIP) assays using antibodies of acetylated histone H3 were performed in jejunum of mice fed a high carbohydrate/fat ratio diet or a low carbohydrate/fat ratio for 7 d. Results: The acetylations of histone H3 of lysine 9/14 were highly detected on the SGLT1 gene and the levels were enhanced by feeding mice a high carbohydrate/fat ratio diet. Histone H3 acetylation at lysine 9 was enhanced by feeding a high carbohydrate/fat ratio diet, whereas that at lysine 14 was not enhanced significantly. Conclusion: These observations indicate that induction of SGLT1 gene expression by feeding a high carbohydrate/fat ratio diet is associated with acetylation of histone H3 at lysine 9 on the SGLT I gene. (C) 2009 Published by Elsevier Inc.
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