期刊
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
卷 27, 期 3, 页码 261-278出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15257770701853679
关键词
pseudothymidine; C-glycoside; polymerase; DNA
资金
- NHGRI NIH HHS [R21 HG003581, R21 HG003581-02, HG 003581] Funding Source: Medline
Thermal denaturation and circular dichroism studies suggested that multiple (up to 12), sequential pseudothymidines, a representative C-glycoside, do not perturb the structure of a representative DNA duplex. Further, various Family A and B DNA polymerases were found to extend a primer by incorporating four sequential pseudothymidine triphosphates, and then continue the extension to generate full-length product. Detailed studies showed that Taq polymerase incorporated up to five sequential C-glycosides, but not more. These results constrain architectures for sequencing, quantitating, and analyzing DNA analogs that exploit C-glycosides, and define better the challenge of creating a synthetic biology using these with natural polymerases.
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