期刊
NUCLEIC ACIDS RESEARCH
卷 42, 期 13, 页码 8565-8577出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gku560
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资金
- National Institutes of Health [R01 GM099705, T32 GM08759]
- Howard Hughes Medical Institute
- University of Colorado Boulder Open Access Fund
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM099705, T32GM008759] Funding Source: NIH RePORTER
Telomerase is the ribonucleoprotein (RNP) enzyme that elongates telomeric DNA to compensate for the attrition occurring during each cycle of DNA replication. Knowing the levels of telomerase in continuously dividing cells is important for understanding how much telomerase is required for cell immortality. In this study, we measured the endogenous levels of the human telomerase RNP and its two key components, human telomerase RNA (hTR) and human telomerase reverse transcriptase (hTERT). We estimate similar to 240 telomerase monomers per cell for HEK 293T and HeLa, a number similar to that of telomeres in late S phase. The subunits were in excess of RNPs (e.g. similar to 1150 hTR and similar to 500 hTERT molecules per HeLa cell), suggesting the existence of unassembled components. This hypothesis was tested by overexpressing individual subunits, which increased total telomerase activity as measured by the direct enzyme assay. Thus, there are subpopulations of both hTR and hTERT not assembled into telomerase but capable of being recruited. We also determined the specific activity of endogenous telomerase and of overexpressed super-telomerase both to be similar to 60 nt incorporated per telomerase per minute, with K-m(dGTP) similar to 17 mu M, indicating super-telomerase is as catalytically active as endogenous telomerase and is thus a good model for biochemical studies.
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