期刊
NUCLEIC ACIDS RESEARCH
卷 42, 期 21, 页码 13422-13429出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gku1122
关键词
-
资金
- California State Polytechnic University
- CSUPERB
- Research Corporation [Cottrell College Science Award] [10484]
- St. John's University
- Army Research Office [Award] [W911NF1110371]
- NSF CAREER Award [DMR1149665]
- University of Maryland startup funds
We have determined the 1.50 angstrom A crystal structure of the DNA decamer, d(CCA(CNV)KGCGTGG) (K-CNV, 3-cyanovinylcarbazole), which forms a G-quadruplex structure in the presence of Ba2+. The structure contains several unique features including a bulged nucleotide and the first crystal structure observation of a C-tetrad. The structure reveals that water molecules mediate contacts between the divalent cations and the C-tetrad, allowing Ba2+ ions to occupy adjacent steps in the central ion channel. One ordered Mg2+ facilitates 3'-3' stacking of two quadruplexes in the asymmetric unit, while the bulged nucleotide mediates crystal contacts. Despite the high diffraction limit, the first four nucleotides including the K-CNV nucleoside are disordered though they are still involved in crystal packing. This work suggests that the bulky hydrophobic groups may locally influence the formation of non-Watson-Crick structures from otherwise complementary sequences. These observations lead to the intriguing possibility that certain types of DNA damage may act as modulators of G-quadruplex formation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据