期刊
NUCLEIC ACIDS RESEARCH
卷 43, 期 2, 页码 1257-1267出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gku1355
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资金
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDB08010203]
- National Natural Science Foundation of China [31325007]
- Beijing Municipal Government
Clustered regularly interspaced short palindromic repeat (CRISPR) loci and CRISPR-associated ( Cas) proteins form an adaptive immune system that protects prokaryotes against plasmids and viruses. The Cmr complex, a type III-B effector complex, uses the CRISPR RNA (crRNA) as a guide to target RNA. Here, we show that the Cmr complex of Pyrococcus furio-sus cleaves RNA at multiple sites that are 6 nt apart and are positioned relative to the 5'-end of the crRNA. We identified Cmr4 as the slicer and determined its crystal structure at 2.8 A resolution. In the crystal, Cmr4 forms a helical filament that most likely reflects its structural organization in the Cmr complex. The putative active site is located at the inner surface of the filament where the guide and substrate RNA are thought to bind. The filament structure of Cmr4 accounts for multiple periodic cleavage sites on the substrate. Our study provides new insights into the structure and mechanism of the RNA-targeting Cmr complex.
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