期刊
NUCLEIC ACIDS RESEARCH
卷 42, 期 9, 页码 5416-5425出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gku202
关键词
-
资金
- National Institutes of Health/National Cancer Institute [R01-CA135030, P01-CA081534, U01-CA152758, U01-CA166905]
- U.S. National Science Foundation [DBI-0445666, DBI-0421604, NSF IOS-0922738, MCB-0929339, N2010 IOB-0519985]
- National Institutes of Health [2R01GM032877-25A1]
RNAi is a powerful tool for the regulation of gene expression. It is widely and successfully employed in functional studies and is now emerging as a promising therapeutic approach. Several RNAi-based clinical trials suggest encouraging results in the treatment of a variety of diseases, including cancer. Here we present miR-Synth, a computational resource for the design of synthetic microRNAs able to target multiple genes in multiple sites. The proposed strategy constitutes a valid alternative to the use of siRNA, allowing the employment of a fewer number of molecules for the inhibition of multiple targets. This may represent a great advantage in designing therapies for diseases caused by crucial cellular pathways altered by multiple dysregulated genes. The system has been successfully validated on two of the most prominent genes associated to lung cancer, c-MET and Epidermal Growth Factor Receptor (EGFR). (See http://microrna.osumc.edu/mir-synth).
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据