期刊
NUCLEIC ACIDS RESEARCH
卷 42, 期 8, 页码 4962-4971出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gku168
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资金
- ANRS
- Sidaction, Institut Universitaire de France
- Genopole Evry
- Institut des Hautes Etudes Scientifiques
- Centre National de la Recherche Scientifique (CNRS)
- FRS-FNRS (Belgium)
- Televie-Programme of the FRS-FNRS
- Programme d'Excellence Cibles of the Walloon region
- NEAT (European AIDS treatment network) integration grant
- International Brachet Stiftung
- Fondation Roi Baudouin (Belgium)
- Centre National de la Recherche Scientifique
Active positive transcription elongation factor b (P-TEFb) is essential for cellular and human immunodeficiency virus type 1 (HIV-1) transcription elongation. CTIP2 represses P-TEFb activity in a complex containing 7SK RNA and HEXIM1. Recently, the inactive 7SK/P-TEFb small nuclear RNP (snRNP) has been detected at the HIV-1 core promoter as well as at the promoters of cellular genes, but a recruiting mechanism still remains unknown to date. Here we show global synergy between CTIP2 and the 7SK-binding chromatin master-regulator HMGA1 in terms of P-TEFb-dependent endogenous and HIV-1 gene expression regulation. While CTIP2 and HMGA1 concordingly repress the expression of cellular 7SK-dependent P-TEFb targets, the simultaneous knock-down of CTIP2 and HMGA1 also results in a boost in Tat-dependent and independent HIV-1 promoter activity. Chromatin immunoprecipitation experiments reveal a significant loss of CTIP2/7SK/P-TEFb snRNP recruitment to cellular gene promoters and the HIV-1 promoter on HMGA1 knock-down. Our findings not only provide insights into a recruiting mechanism for the inactive 7SK/P-TEFb snRNP, but may also contribute to a better understanding of viral latency.
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