期刊
NUCLEIC ACIDS RESEARCH
卷 42, 期 8, 页码 5097-5108出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gku147
关键词
-
资金
- National Institutes of Health (NIH) [GM21941]
During initial transcription, RNA polymerase remains bound at the promoter and synthesizes RNA without movement along the DNA template, drawing downstream DNA into itself in a process called scrunching and thereby storing energy to sever the bonds that hold the enzyme at the promoter. We show that DNA scrunching also is the driving force behind the escape of RNA polymerase from a regulatory pause of the late gene operon of bacteriophage lambda, and that this process is enhanced by the activity of the Q(lambda) antiterminator. Furthermore, we show that failure of transcription complexes to escape the pause results in backtracking and arrest in a process analogous to abortive initiation. We identify a sequence element that modulates both abortive synthesis and the formation of arrested elongation complexes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据