期刊
NUCLEIC ACIDS RESEARCH
卷 42, 期 15, 页码 9730-9739出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gku717
关键词
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资金
- Ministry of Science and Technology of China [2010CB944901, 2011CB965104, 2012AA020405]
- National Natural Science Foundation of China [91019017, 31271373, 31200952]
- Aurora Talent Project of Shanghai [10SG24]
- Program for Eastern Scholar of Shanghai
- Fundamental Research Funds for the Central Universities [20113048, 20113109]
- Ministry of Science and Technology of China
ATP-dependent chromatin remodeling complexes regulate nucleosome organizations. In Drosophila, gene Brm encodes the core Brahma complex, the ATPase subunit of SWI/SNF class of chromatin remodelers. Its role in modulating the nucleosome landscape in vivo is unclear. In this study, we knocked down Brm in Drosophila third instar larvae to explore the changes in nucleosome profiles and global gene transcription. The results show that Brm knockdown leads to nucleosome occupancy changes throughout the entire genome with a bias in occupancy decrease. In contrast, the knockdown has limited impacts on nucleosome position shift. The knockdown also alters another important physical property of nucleosome positioning, fuzziness. Nucleosome position shift, gain or loss and fuzziness changes are all enriched in promoter regions. Nucleosome arrays around the 5' ends of genes are reorganized in five patterns as a result of Brm knockdown. Intriguingly, the concomitant changes in the genes adjacent to the Brahma-dependent remodeling regions have important roles in development and morphogenesis. Further analyses reveal abundance of AT-rich motifs for transcription factors in the remodeling regions.
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