期刊
NUCLEIC ACIDS RESEARCH
卷 43, 期 2, 页码 787-802出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gku1349
关键词
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资金
- Spanish Ministry of Economy and Competitiveness and European Union funds (FEDER) [BFU2010-21975-C03-01, BFU2010-21975-C03-03]
- Regional Valencian Government [PROMETEO 2011/088, ACOMP/2014/253]
- Spanish Ministry of Science and Innovation [BES-2008-009625, BES-2008-003969FP]
- European Science Foundation Short-Term Travelling Grant
- La Fundacion para la Investigacion y la Prevencion del Sida en Espana [360946/10]
- Regional Andalusian Government [P12-BIO1938MO]
- European Molecular Biology Laboratory
- Deutsche Forschungsgemeinschaft [1422/3-1]
- National Institutes of Health [R01 GM068717]
- Spanish MINECO
- European Union Funds (FEDER) [BFU2013-48643-C3-3-P]
The particular behaviour of eukaryotic RNA poly-merases along different gene regions and amongst distinct gene functional groups is not totally understood. To cast light onto the alternative active or backtracking states of RNA polymerase II, we have quantitatively mapped active RNA polymerases at a high resolution following a new biotin-based genomic run-on (BioGRO) technique. Compared with conventional profiling with chromatin immunopre-cipitation, the analysis of the BioGRO profiles in Sac-charomyces cerevisiae shows that RNA polymerase II has unique activity profiles at both gene ends, which are highly dependent on positioned nucleosomes. This is the first demonstration of the in vivo influence of positioned nucleosomes on transcription elongation. The particular features at the 5' end and around the polyadenylation site indicate that this polymerase undergoes extensive specific-activity regulation in the initial and final transcription elongation phases. The genes encoding for ribosomal proteins show distinctive features at both ends. BioGRO also provides the first nascentome analysis for RNA polymerase III, which indicates that transcription of tRNA genes is poorly regulated at the individual copy level. The present study provides a novel perspective of the transcription cycle that incorporates inactivation/reactivation as an important aspect of RNA polymerase dynamics.
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