期刊
NUCLEIC ACIDS RESEARCH
卷 42, 期 2, 页码 1052-1064出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkt932
关键词
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资金
- Ministry of Science and Technology of China [2009CB919000, 2011CBA00906, 2012CB316500, 2012DFG32160]
- National Natural Science Foundation of China [30971431, 31000365, 61273228, 31100068, 91019016]
- Grants-in-Aid for Scientific Research [24390045] Funding Source: KAKEN
Rhodococcus opacus strain PD630 (R. opacus PD630), is an oleaginous bacterium, and also is one of few prokaryotic organisms that contain lipid droplets (LDs). LD is an important organelle for lipid storage but also intercellular communication regarding energy metabolism, and yet is a poorly understood cellular organelle. To understand the dynamics of LD using a simple model organism, we conducted a series of comprehensive omics studies of R. opacus PD630 including complete genome, transcriptome and proteome analysis. The genome of R. opacus PD630 encodes 8947 genes that are significantly enriched in the lipid transport, synthesis and metabolic, indicating a super ability of carbon source biosynthesis and catabolism. The comparative transcriptome analysis from three culture conditions revealed the landscape of gene-altered expressions responsible for lipid accumulation. The LD proteomes further identified the proteins that mediate lipid synthesis, storage and other biological functions. Integrating these three omics uncovered 177 proteins that may be involved in lipid metabolism and LD dynamics. A LD structure-like protein LPD06283 was further verified to affect the LD morphology. Our omics studies provide not only a first integrated omics study of prokaryotic LD organelle, but also a systematic platform for facilitating further prokaryotic LD research and biofuel development.
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