期刊
NUCLEIC ACIDS RESEARCH
卷 40, 期 W1, 页码 W209-W213出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gks396
关键词
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资金
- Michael Smith Foundation for Health Research
- Canadian Institute of Health Research
Restriction-free cloning (RF-cloning) is a PCR-based technology that expands on the QuikChange (TM) mutagenesis process originally popularized by Stratagene in the mid-1990s, and allows the insertion of essentially any sequence into any plasmid at any location. While RF-cloning is a powerful tool for the design of custom plasmids when restriction sites are not conveniently situated, manually designing the requisite primers can be tedious and error prone. We present here a web-service that automates the primer design process, along with a user interface that includes a number of useful tools for managing both the input sequences and the resulting outputs. RF-Cloning is free and open to all users, and can be accessed at http://www.rf-cloning.org.
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