期刊
NUCLEIC ACIDS RESEARCH
卷 41, 期 3, 页码 1984-1997出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gks1309
关键词
-
资金
- National Research Foundation of Korea by the Korean Government MEST [2011-0027376, 2012R1A1A2008826]
- Next-Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea [PJ009104]
- Sogang University Research [201214003.01]
- UNIK research initiative of the Danish Ministry of Science, Technology and Innovation [09-065274]
- Chinese Academy of Sciences
- National Institutes of Health [R01GM070795, R01GM059138]
- USDA [2008-35100-04518]
- University of California at Riverside Initial Complement Fund
- United States Department of Agriculture hatch fund [CA-R-BPS-7754H]
- National Research Foundation of Korea [2012R1A1A2008826] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
MicroRNAs (miRNAs) are small regulatory RNAs that have important regulatory roles in numerous developmental and metabolic processes in most eukaryotes. In Arabidopsis, DICER-LIKE1 (DCL1), HYPONASTIC LEAVES 1, SERRATE, HUA ENHANCER1 and HASTY are involved in processing of primary miRNAs (pri-miRNAs) to yield precursor miRNAs (pre-miRNAs) and eventually miRNAs. In addition to these components, mRNA cap-binding proteins, CBP80/ABA HYPERSENSITIVE1 and CBP20, also participate in miRNA biogenesis. Here, we show that STABILIZED1 (STA1), an Arabidopsis pre-mRNA processing factor 6 homolog, is also involved in the biogenesis of miRNAs. Similar to other miRNA biogenesis-defective mutants, sta1-1 accumulated significantly lower levels of mature miRNAs and concurrently higher levels of pri-miRNAs than wild type. The dramatic reductions of mature miRNAs were associated with the accumulation of their target gene transcripts and developmental defects. Furthermore, sta1-1 impaired splicing of intron containing pri-miRNAs and decreased transcript levels of DCL1. These results suggest that STA1 is involved in miRNA biogenesis directly by functioning in pri-miRNA splicing and indirectly by modulating the DCL1 transcript level.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据