期刊
NUCLEIC ACIDS RESEARCH
卷 40, 期 4, 页码 1499-1508出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkr882
关键词
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资金
- Biological Sciences Research Council (BBSRC)
- Cancer Research UK
- Deutscher Akademischer Austausch Dienst (DAAD)
- BBSRC [BB/E012752/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/E012752/1] Funding Source: researchfish
- Cancer Research UK [11961, 12488] Funding Source: researchfish
SP1 is a ubiquitous transcription factor that is involved in the regulation of various house-keeping genes. It is known that it acts by binding to a double-stranded consensus motif. Here, we have discovered that SP1 binds also to a non-canonical DNA structure, a G-quadruplex, with high affinity. In particular, we have studied the SP1 binding site within the promoter region of the c-KIT oncogene and found that this site can fold into an anti-parallel two-tetrad G-quadruplex. SP1 pull-down experiments from cellular extracts, together with biophysical binding assays revealed that SP1 has a comparable binding affinity for this G-quadruplex structure and the canonical SP1 duplex sequence. Using SP1 ChIP-on-chip data sets, we have also found that 87% of SP1 binding sites overlap with G-quadruplex forming sequences. Furthermore, while many of these immuoprecipitated sequences (36%) even lack the minimal SP1 consensus motif, 5'-GGGCGG-3', we have shown that 77% of them are putative G-quadruplexes. Collectively, these data suggest that SP1 is able to bind both, canonical SP1 duplex DNA as well as G-quadruplex structures in vitro and we hypothesize that both types of interactions may occur in cells.
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