期刊
NUCLEIC ACIDS RESEARCH
卷 39, 期 20, 页码 8984-8991出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkr540
关键词
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资金
- Singapore Ministry of Education [ARC30/07, RG62/07]
- Singapore Biomedical Research Council [07/1/22/19/542]
- Oxford University Press
T30177 is a G-rich oligonucleotide with the sequence (GTGGTGGGTGGGTGGGT) which inhibits the HIV-1 integrase activity at nanomolar concentrations. Here we show that this DNA sequence forms in K+ solution a dimeric G-quadruplex structure comprising a total of six G-tetrad layers through the stacking of two propeller-type parallel-stranded G-quadruplex subunits at their 5'-end. All twelve guanines in the sequence participate in the G-tetrad formation, despite the interruption in the first G-tract by a thymine, which forms a bulge between two adjacent G-tetrads. In this work, we also propose a simple analytical approach to stoichiometry determination using concentration-dependent melting curves.
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