期刊
NUCLEIC ACIDS RESEARCH
卷 39, 期 13, 页码 5538-5554出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkr124
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资金
- Higo Bank
- Cooperative Research Project on Clinical and Epidemiological Studies of Emerging and Re-emerging Infectious Diseases
- Japan Society for the Promotion of Science
- Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT)
- Research and Education Program for Development of Therapy of Emerging and Re-emerging Infectious Diseases Including AIDS
- Global Centers of Excellence (COE), Global Education Research Center Aiming at the Control of AIDS
- Grants-in-Aid for Scientific Research [23590546, 22590428] Funding Source: KAKEN
The ability of mammalian cytidine deaminases encoded by the APOBEC3 (A3) genes to restrict a broad number of endogenous retroelements and exogenous retroviruses, including murine leukemia virus and human immunodeficiency virus (HIV)-1, is now well established. The RNA editing family member apolipoprotein B (apo B)-editing catalytic subunit 1 (APOBEC1; A1) from a variety of mammalian species, a protein involved in lipid transport and which mediates C-U deamination of mRNA for apo B, has also been shown to modify a range of exogenous retroviruses, but its activity against endogenous retroelements remains unclear. Here, we show in cell culture-based retrotransposition assays that A1 family proteins from multiple mammalian species can also reduce the mobility and infectivity potential of LINE-1 (long interspersed nucleotide sequence-1, L1) and long-terminal repeats (LTRs) retrotransposons (or endogenous retroviruses), such as murine intracisternal A-particle (IAP) and MusD sequences. The anti-L1 activity of A1 was mainly mediated by a deamination-independent mechanism, and was not affected by subcellular localization of the proteins. In contrast, the inhibition of LTR-retrotransposons appeared to require the deaminase activity of A1 proteins. Thus, the AID/APOBEC family proteins including A1s employ multiple mechanisms to regulate the mobility of autonomous retrotransposons in several mammalian species.
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