期刊
NUCLEIC ACIDS RESEARCH
卷 39, 期 12, 页码 4975-4983出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkr079
关键词
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资金
- US National Cancer Institute [P01 CA77852]
- Basic and Cancer Immunology Training Grant [CA009537]
- US National Institutes of Health [R01 GM41712]
- NIH [R01 GM65988]
- Cancer Research Institute
- National Cancer Institute, Center for Cancer Research [Z01 BC 006150-19LMP]
The RNA Pol II transcription complex pauses just downstream of the promoter in a significant fraction of human genes. The local features of genomic structure that contribute to pausing have not been defined. Here, we show that genes that pause are more G-rich within the region flanking the transcription start site (TSS) than RefSeq genes or non-paused genes. We show that enrichment of binding motifs for common transcription factors, such as SP1, may account for G-richness upstream but not downstream of the TSS. We further show that pausing correlates with the presence of a GrIn1 element, an element bearing one or more G4 motifs at the 5'-end of the first intron, on the non-template DNA strand. These results suggest potential roles for dynamic G4 DNA and G4 RNA structures in cis-regulation of pausing, and thus genome-wide regulation of gene expression, in human cells.
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