期刊
NUCLEIC ACIDS RESEARCH
卷 40, 期 7, 页码 3218-3231出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkr1139
关键词
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资金
- Ministry of Education, Science, Sports and Culture of Japan [22121517, 23657072, 20570111, 23570146, 21370047]
- Yokohama City University [T2203]
- Advanced Medical Research Center of Yokohama City University [II (10), S2210]
- Yokohama Academic Foundation
- Japan Science and Technology (SENTAN)
- Naito Foundation
- Sumitomo Denko Foundation
- Iwatani Foundation
- MEXT [21370047]
- Grants-in-Aid for Scientific Research [23657072, 21370047, 20570111, 24121714, 22121517, 23570146, 22570116] Funding Source: KAKEN
Mammalian Musashi1 (Msi1) is an RNA-binding protein that regulates the translation of target mRNAs, and participates in the maintenance of cell 'stemness' and tumorigenesis. Msi1 reportedly binds to the 3'-untranslated region of mRNA of Numb, which encodes Notch inhibitor, and impedes initiation of its translation by competing with eIF4G for PABP binding, resulting in triggering of Notch signaling. Here, the mechanism by which Msi1 recognizes the target RNA sequence using its Ribonucleoprotein (RNP)-type RNA-binding domains (RBDs), RBD1 and RBD2 has been revealed on identification of the minimal binding RNA for each RBD and determination of the three-dimensional structure of the RBD1:RNA complex. Unique interactions were found for the recognition of the target sequence by Msi1 RBD1: adenine is sandwiched by two phenylalanines and guanine is stacked on the tryptophan in the loop between beta 1 and alpha 1. The minimal recognition sequences that we have defined for Msi1 RBD1 and RBD2 have actually been found in many Msi1 target mRNAs reported to date. The present study provides molecular clues for understanding the biology involving Musashi family proteins.
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