期刊
NUCLEIC ACIDS RESEARCH
卷 39, 期 3, 页码 949-957出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkq889
关键词
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资金
- Biotechnology and Biological Sciences Research Council [BB/G005915/1, BB/E0020690]
- MRC [G0800970]
- Leverhulme Trust
- Biotechnology and Biological Sciences Research Council [BB/E020690/1, BB/G005915/1] Funding Source: researchfish
- Medical Research Council [G0800970] Funding Source: researchfish
- BBSRC [BB/E020690/1, BB/G005915/1] Funding Source: UKRI
- MRC [G0800970] Funding Source: UKRI
Genome duplication requires accessory helicases to displace proteins ahead of advancing replication forks. Escherichia coli contains three helicases, Rep, UvrD and DinG, that might promote replication of protein-bound DNA. One of these helicases, Rep, also interacts with the replicative helicase DnaB. We demonstrate that Rep is the only putative accessory helicase whose absence results in an increased chromosome duplication time. We show also that the interaction between Rep and DnaB is required for Rep to maintain rapid genome duplication. Furthermore, this Rep-DnaB interaction is critical in minimizing the need for both recombinational processing of blocked replication forks and replisome reassembly, indicating that colocalization of Rep and DnaB minimizes stalling and subsequent inactivation of replication forks. These data indicate that E. coli contains only one helicase that acts as an accessory motor at the fork in wild-type cells, that such an activity is critical for the maintenance of rapid genome duplication and that colocalization with the replisome is crucial for this function. Given that the only other characterized accessory motor, Saccharomyces cerevisiae Rrm3p, associates physically with the replisome, our demonstration of the functional importance of such an association indicates that colocalization may be a conserved feature of accessory replicative motors.
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