期刊
NUCLEIC ACIDS RESEARCH
卷 38, 期 15, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkq543
关键词
-
资金
- National Institutes of Health [5R01HG003710-02]
- Pacific Biosciences
A novel template design for single-molecule sequencing is introduced, a structure we refer to as a SMRTbell (TM) template. This structure consists of a double-stranded portion, containing the insert of interest, and a single-stranded hairpin loop on either end, which provides a site for primer binding. Structurally, this format resembles a linear double-stranded molecule, and yet it is topologically circular. When placed into a single-molecule sequencing reaction, the SMRTbell template format enables a consensus sequence to be obtained from multiple passes on a single molecule. Furthermore, this consensus sequence is obtained from both the sense and antisense strands of the insert region. In this article, we present a universal method for constructing these templates, as well as an application of their use. We demonstrate the generation of high-quality consensus accuracy from single molecules, as well as the use of SMRTbell templates in the identification of rare sequence variants.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据