期刊
NUCLEIC ACIDS RESEARCH
卷 39, 期 1, 页码 89-103出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkq740
关键词
-
资金
- Direccion General de Asuntos del Personal Academico-Universidad Nacional Autonoma de Mexico [IN209403, IN214407]
- Consejo Nacional de Ciencia y Tecnologia [CONACyT: 42653-Q, 58767]
- CONACyT [170087, 207989]
- Direccion General de estudios de Posgrado-Universidad Nacional Autonoma de Mexico (DGEP)
- UNAM
- Spanish Ministry of Education and Science [BFU2006-12185]
- Spanish Ministry of Science and Innovation [BIO2009-12697]
- Universidad Nacional Autonoma de Mexico (UNAM), Instituto de Fisiologia Celular
Genome organization into transcriptionally active domains denotes one of the first levels of gene expression regulation. Although the chromatin domain concept is generally accepted, only little is known on how domain organization impacts the regulation of differential gene expression. Insulators might hold answers to address this issue as they delimit and organize chromatin domains. We have previously identified a CTCF-dependent insulator with enhancer-blocking activity embedded in the 5' non-coding region of the chicken alpha-globin domain. Here, we demonstrate that this element, called the alpha EHS-1.4 insulator, protects a transgene against chromosomal position effects in stably transfected cell lines and transgenic mice. We found that this insulator can create a regulated chromatin environment that coincides with the onset of adult alpha-globin gene expression. Furthermore, such activity is in part dependent on the in vivo regulated occupancy of CTCF at the alpha EHS-1.4 element. Insulator function is also regulated by CTCF poly(ADP-ribosyl)ation. Our results suggest that the alpha EHS-1.4 insulator contributes in organizing the chromatin structure of the alpha-globin gene domain and prevents activation of adult alpha-globin gene expression at the erythroblast stage via CTCF.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据