期刊
NUCLEIC ACIDS RESEARCH
卷 37, 期 20, 页码 6984-6990出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkp687
关键词
-
资金
- Synthetic Genomics, Inc.
Here it is demonstrated that the yeast Saccharomyces cerevisiae can take up and assemble at least 38 overlapping single-stranded oligonucleotides and a linear double-stranded vector in one transformation event. These oligonucleotides can overlap by as few as 20 bp, and can be as long as 200 nucleotides in length. This straightforward scheme for assembling chemically-synthesized oligonucleotides could be a useful tool for building synthetic DNA molecules.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据