期刊
NUCLEIC ACIDS RESEARCH
卷 37, 期 12, 页码 4157-4169出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkp314
关键词
-
资金
- Ministerio de Ciencia e Innovacion, Direccion General de Investigacion (MCI-DGI) [BFU2009-07167]
- US National Institutes of Health [GM48804]
In the presence of Mn2+, an activity in a preparation of purified Bacillus subtilis RecN degrades single-stranded (ss) DNA with a 3' -> 5' polarity. This activity is not associated with RecN itself, because RecN purified from cells lacking polynucleotide phosphorylase (PNPase) does not show the exonuclease activity. We show here that, in the presence of Mn2+ and low-level inorganic phosphate (P-i), PNPase degrades ssDNA. The limited end-processing of DNA is regulated by ATP and is inactive in the presence of Mg2+ or high-level P-i. In contrast, the RNase activity of PNPase requires Mg2+ and P-i, suggesting that PNPase degradation of RNA and ssDNA occur by mutually exclusive mechanisms. A null pnpA mutation (delta pnpA) is not epistatic with delta recA, but is epistatic with delta recN and delta ku, which by themselves are non-epistatic. The addA5, delta recO, delta recQ (delta recJ), delta recU and delta recG mutations (representative of different epistatic groups), in the context of delta pnpA, demonstrate gain- or loss-of-function by inactivation of repair-by-recombination, depending on acute or chronic exposure to the damaging agent and the nature of the DNA lesion. Our data suggest that PNPase is involved in various nucleic acid metabolic pathways, and its limited ssDNA exonuclease activity plays an important role in RecA-dependent and RecA-independent repair pathways.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据