期刊
NUCLEIC ACIDS RESEARCH
卷 37, 期 11, 页码 3531-3544出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkp214
关键词
-
资金
- Ministry of Education, Culture, Sports, Science, and Technology (MEXT)
- Naito foundation
- Japan Society for the Promotion of Science (JSPS) [19790316, 19657002, 1770001]
- University of Tokyo
- Grants-in-Aid for Scientific Research [19790316, 19657002] Funding Source: KAKEN
Cleavage of a DNA replication fork leads to fork restoration by recombination repair. In prokaryote cells carrying restrictionmodification systems, fork passage reduces genome methylation by the modification enzyme and exposes the chromosome to attack by the restriction enzyme. Various observations have suggested a relationship between the fork and Type I restriction enzymes, which cleave DNA at a distance from a recognition sequence. Here, we demonstrate that a Type I restriction enzyme preparation cleaves a model replication fork at its branch. The enzyme probably tracks along the DNA from an unmethylated recognition site on the daughter DNA and cuts the fork upon encountering the branch point. Our finding suggests that these restrictionmodification systems contribute to genome maintenance through cell death and indicates that DNA replication fork cleavage represents a critical point in genome maintenance to choose between the restoration pathway and the destruction pathway.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据