期刊
NUCLEIC ACIDS RESEARCH
卷 38, 期 2, 页码 441-454出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkp905
关键词
-
资金
- Medical Research Council
- Biotechnology and Biological Sciences Research Council [BB\E015662/1]
- North West Cancer Research Fund [CR782]
- National Cancer Institute [CA40615]
- Lancaster University
- BBSRC [BB/E015662/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/E015662/1] Funding Source: researchfish
- NATIONAL CANCER INSTITUTE [R01CA040615] Funding Source: NIH RePORTER
The repair of DNA double-strand breaks (DSBs) is essential to maintain genomic integrity. In higher eukaryotes, DNA DSBs are predominantly repaired by non-homologous end joining (NHEJ), but DNA ends can also be joined by an alternative error-prone mechanism termed microhomology-mediated end joining (MMEJ). In MMEJ, the repair of DNA breaks is mediated by annealing at regions of microhomology and is always associated with deletions at the break site. In budding yeast, the Mre11/Rad5/Xrs2 complex has been demonstrated to play a role in both classical NHEJ and MMEJ, but the involvement of the analogous MRE11/RAD50/NBS1 (MRN) complex in end joining in higher eukaryotes is less certain. Here we demonstrate that in Xenopus laevis egg extracts, the MRN complex is not required for classical DNA-PK-dependent NHEJ. However, the XMRN complex is necessary for resection-based end joining of mismatched DNA ends. This XMRN-dependent end joining process is independent of the core NHEJ components Ku70 and DNA-PK, occurs with delayed kinetics relative to classical NHEJ and brings about repair at sites of microhomology. These data indicate a role for the X. laevis MRN complex in MMEJ.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据