期刊
NUCLEIC ACIDS RESEARCH
卷 37, 期 17, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkp539
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资金
- The Hungarian Scientific Research Fund (OTKA) [TO48742, 72762]
- Ministry of Public Health ETT [067/2006]
- Hungarian Academy of Sciences
Double-stranded (ds), as well as denatured, single-stranded (ss) DNA samples can be analyzed on urea-agarose gels. Here we report that after denaturation by heat in the presence of 8 M urea, the two strands of the same ds DNA fragment of similar to 1-20-kb size migrate differently in 1 M urea containing agarose gels. The two strands are readily distinguished on Southern blots by ss-specific probes. The different migration of the two strands could be attributed to their different, base composition-dependent conformation impinging on the electrophoretic mobility of the ss molecules. This phenomenon can be exploited for the efficient preparation of strand-specific probes and for the separation of the complementary DNA strands for subsequent analysis, offering a new tool for various cell biological research areas.
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