期刊
NUCLEIC ACIDS RESEARCH
卷 37, 期 6, 页码 1907-1914出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkp050
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资金
- JSPS
- MEXT
- Mitsubishi Foundation
- Grants-in-Aid for Scientific Research [20112007] Funding Source: KAKEN
U1 snRNP plays a crucial role in the 5 splice site recognition during splicing. Here we report the first example of naturally occurring U1-independent U2-type splicing in humans. The U1 components were not included in the pre-spliceosomal E complex formed on the human F1 (hF1) intron 9 in vitro. Moreover, hF1 intron 9 was efficiently spliced even in U1-disrupted Xenopus oocytes as well as in U1-inactivated HeLa nuclear extracts. Finally, hF1 exon 9 skipping induced by an alternative splicing regulator Fox-1 was impaired when intron 9 was changed to the U1-dependent one. Our results suggest that U1-independent splicing contributes to the regulation of alternative splicing of a class of pre-mRNAs.
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