期刊
NUCLEIC ACIDS RESEARCH
卷 36, 期 11, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkn210
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资金
- NIAAA NIH HHS [R01AA016676, R01 AA016676] Funding Source: Medline
- NIA NIH HHS [P01 AG020641, P01AG20641] Funding Source: Medline
DNA methylation at cytosines is a widely studied epigenetic modification. Methylation is commonly detected using bisulfite modification of DNA followed by PCR and additional techniques such as restriction digestion or sequencing. These additional techniques are either laborious, require specialized equipment, or are not quantitative. Here we describe a simple algorithm that yields quantitative results from analysis of conventional four-dye-trace sequencing. We call this method Mquant and we compare it with the established laboratory method of combined bisulfite restriction assay (COBRA). This analysis of sequencing electropherograms provides a simple, easily applied method to quantify DNA methylation at specific CpG sites.
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