期刊
NUCLEIC ACIDS RESEARCH
卷 37, 期 2, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkn932
关键词
-
资金
- Pacific Ovarian Cancer Research Consortium (POCRC)/Ovarian SPORE [P50 CA83636]
- FHCRC Molecular Diagnostics Program
- POCRC/Ovarian SPORE [P50 CA83636]
- National Human Genome Research Institute [5P41 HG004059]
- Human Frontier Science Program [RGP0022/2005]
MicroRNAs are small (22 nt) RNAs that regulate gene expression and play important roles in both normal and disease physiology. The use of microarrays for global characterization of microRNA expression is becoming increasingly popular and has the potential to be a widely used and valuable research tool. However, microarray profiling of microRNA expression raises a number of data analytic challenges that must be addressed in order to obtain reliable results. We introduce here a universal reference microRNA reagent set as well as a series of nonhuman spiked-in synthetic microRNA controls, and demonstrate their use for quality control and between-array normalization of microRNA expression data. We also introduce diagnostic plots designed to assess and compare various normalization methods. We anticipate that the reagents and analytic approach presented here will be useful for improving the reliability of microRNA microarray experiments.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据