期刊
NUCLEIC ACIDS RESEARCH
卷 36, 期 21, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkn735
关键词
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资金
- National Cancer Institute of Canada
- Canadian Cancer Society [018298]
- Canadian Institutes of Health Research [MOP 67048]
- Canada Research Chairs Program
- Knudson Postdoctoral Fellowship
- PreCarn Foundation
- Natural Sciences and Engineering Research Council of Canada
- Excellence In Radiation Research for the 21st Century Program
- Terry Fox Foundation Fellowship
- Ontario Graduate Scholarships
High-throughput, microarray-based chromatin immunoprecipitation (ChIP-chip) technology allows in vivo elucidation of transcriptional networks. However this complex is not yet readily accessible, in part because its many parameters have not been systematically evaluated and optimized. We address this gap by systematically assessing experimental-design parameters including antibody purity, dye-bias, array-batch, inter-day hybridization bias, amplification method and choice of hybridization control. The combined performance of these optimized parameters shows a 90 validation rate in ChIP-chip analysis of Myc genomic binding in HL60 cells using two different microarray platforms. Increased sensitivity and decreased noise in ChIP-chip assays will enable wider use of this methodology to accurately and affordably elucidate transcriptional networks.
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