期刊
NUCLEAR MEDICINE AND BIOLOGY
卷 39, 期 3, 页码 325-333出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.nucmedbio.2011.09.010
关键词
Indium-111; DO3A; Homoalanine; SPECT; Amino acid; LAT; DOTA; Amino acid transporter
资金
- Converging Research Center [2011K000709]
- NRL from MEST [ROA-2008-000-20116-0]
Purpose: We evaluated new In-111-labeled amino acid derivatives, in which the amino acids are conjugated with1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA), 1,4,7,10-tetraazacyclododecane-1,7-diacetic acid (DO2A) or 1,4,7,10-tetraazacyclododectine-1,4,7-triacetic acid (DO3A). Methods: DOTA-aminoalanine (DOTA-A), DOTA-aminohomoalanine (DOTA-H), DOTA-lysine (DOTA-L), DO2A-alanine (DO2A-A), DO3A-alanine (DO3A-A) and DO3A-homoalanine (DO3A-H) were labeled with I In. In vitro cell uptake assays were performed usingHep3B (a human hepatoma cell line), CT26 (a mouse colon cancer cell line) and U87MG (a human glioma cell line). In vitro cell uptake inhibition assays were performed using U87MG and In-111-DO3A-H. U87MG bearing xenografted mice were subject to biodistribution, SPECT imaging, autoradiography, and immunohistochemistry studies. Results: Of the amino acid derivatives and cell lines examined, U87MG and In-111-DO3A-14 showed highest uptake in vitro. This uptake was blocked by 2-aminobicyclo-[2,2,1] heptane-2-carboxylic acid (BCH) and by tryptophan. In-111-DO3A-HSPECT imaging of U87MG bearing xenografted mice visualized tumors (mean tumor-to-muscle ratio 3.16 +/- 0.74). Autoradiography and immunohistochemistry revealed that In-111-DO3A-H uptake matched L-type amino acid transporter 1 expression. Conclusion: Tumor uptake was successfully imaged using In-111-DO3A-H in U87MG bearing xenografted mice. In-111-DO3A-H appears to be useful for imaging tumors expressing L-type amino acid transporter. (C) 2012 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据