期刊
SURFACE & COATINGS TECHNOLOGY
卷 271, 期 -, 页码 2-7出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.surfcoat.2015.02.018
关键词
Hollow Fe3O4/SiO2 composite particles; Polymer nanocomposite microspheres; Pectinase; Enzyme immobilization
The aims of the present work were to establish the magnetite core based nanometer microspheres of morphology concept of multifunctional core/shell nanometer microspheres which had the potential use as pectinase supporter. We had described the convenient method of synthesis and characterization of biodegradable/biocompatible Fe3O4/SiO2@CMCS. The larger aspect ratios of hollow materials could be taken up by cells in larger amounts and have higher internalization rates, which was of great significance for the enhancement of chemotherapy effectiveness. We investigated the enzymatic activity of pectinase adsorbing on novel hollow magnetic composite microspheres. The immobilized enzyme retained more than 50% of its initial activity over 40 days, and the optimum temperature/pH also increased to the range of 50-60 degrees C/3.0-5.0. The immobilized enzyme exhibited great operational stability, and more than 75% residual activity was observed after 10 batch reactions. Moreover, the Fe3O4/SiO2@Carboxymethyl chitosan (CMCS) support presented a very simple, mild and time-saving process for enzyme immobilization. This strategy of immobilizing pectinase made expensive enzymes economically viable, strengthening repeated usage of them because of their rapid and easy separation with a magnet. (c) 2015 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据