期刊
NEW ZEALAND JOURNAL OF BOTANY
卷 52, 期 1, 页码 36-47出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/0028825X.2013.846267
关键词
whole-genome re-sequencing; cyanobacteria; Synechocystis sp. PCC 6803; single nucleotide polymorphism; genome mapping
资金
- University of Otago Senior Smeaton Scholarship in Experimental Science, TSC
- University of Otago PhD Scholarship
- New Zealand Marsden Fund [09-UOO-118]
The photoautotrophic cyanobacterium Synechocystis sp. PCC 6803 is a widely used model in genomic research and was the first photosynthetic organism to have its entire genome sequenced. Here, we report the genome sequence for two glucose-tolerant laboratory 'wild-type' strains, GT-O1 and GT-O2, in use at the University of Otago. Using high-throughput genome sequencing techniques and subsequent Sanger sequencing of detected variants, we have identified ten de novo mutations in the two strains, of which six are unique to GT-O2 cells. The presence of these unique mutations highlights the need to know the genomic background of the parent strain when constructing mutants in Synechocystis sp. PCC 6803 strains.
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