期刊
STRUCTURE
卷 23, 期 9, 页码 1692-1704出版社
CELL PRESS
DOI: 10.1016/j.str.2015.07.002
关键词
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资金
- NIH [P41 GM103712, R01 GM099738, P30 DA035778]
- Medical Research Council [MC_U105174197]
- MRC-T
- MRC [MC_U105174197] Funding Source: UKRI
- Medical Research Council [1273975, MC_U105174197] Funding Source: researchfish
Ionotropic glutamate receptors (iGluRs) are tetrameric ion channels that mediate excitatory neurotransmission. Recent structures of alpha-amino-3-hydroxy- 5-methyl-4-isoxazole propionate (AMPA) and N-methyl-D-aspartate (NMDA) receptors permit a comparative analysis of whole-receptor dynamics for the first time. Despite substantial differences in the packing of their two-domain extracellular region, the two iGluRs share similar dynamics, elucidated by elastic network models. Motions accessible to either structure enable conformational interconversion, such as compression of the AMPA receptor toward the more tightly packed NMDA receptor conformation, which has been linked to allosteric regulation. Pivoting motions coupled to concerted rotations of the transmembrane ion channel are prominent between dimers of distal N-terminal domains in the loosely packed AMPA receptor. The occurrence and functional relevance of these motions is verified by cross-linking experiments designed to probe the computationally predicted distance changes. Together with the identification of hotspot residues acting as mediators of allosteric communication, our data provide a glimpse into the dynamic spectrum of iGluRs.
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