期刊
NEW PHYTOLOGIST
卷 221, 期 2, 页码 738-742出版社
WILEY
DOI: 10.1111/nph.15388
关键词
adaptation; bisulfite sequencing; bsRADseq; DNA methylation; ecological genomics; epiGBS; reduced representation bisulfite sequencing (RRBS); response to environment
资金
- Austrian Science Fund (FWF) project [Y661-B16]
- European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant [764965]
- National Science Foundation (USA) [IOS-1556820]
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [1556738] Funding Source: National Science Foundation
Investigating the features and implications of epigenetic mechanisms across the breadth of organisms and ecosystems is important for understanding the ecological relevance of epigenetics. Several cost-effective reduced representation bisulfite sequencing approaches (RRBS) have been recently developed and applied to different organisms that lack a well annotated reference genome. These new approaches improve the assessment of epigenetic diversity in ecological settings and may provide functional insights. We assess here the opportunities and limitations of RRBS in nonmodel plant species. Well thought out experimental designs that include complementary gene expression studies, and the improvement of genomics resources for the target group, promise to maximize the effect of future RRBS studies.
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