期刊
NEW PHYTOLOGIST
卷 221, 期 3, 页码 1303-1316出版社
WILEY
DOI: 10.1111/nph.15479
关键词
alternative oxidase (AOX); diatoms; metabolite profiling; mitochondria; oxidative stress; photosynthesis; transcriptome
资金
- ERC Advanced Award 'Diatomite'
- LouisD Foundation of the Institut de France
- French Government 'Investissements d'Avenir' programme MEMO LIFE [ANR-10-LABX-54]
- French Government 'Investissements d'Avenir' programme PSL* Research University [ANR-1253 11-IDEX-0001-02]
- French Government 'Investissements d'Avenir' programme OCEANOMICS [ANR-11-BTBR-0008]
- Max Planck Society
- National Science Foundation [NSF-MCB-1024913]
- United States Department of Energy Genomics Science program [DE-SC00006719, DE-SC0008593]
- Gordon and Betty Moore Foundation [GBMF3828]
- U.S. Department of Energy (DOE) [DE-SC0008593] Funding Source: U.S. Department of Energy (DOE)
Diatom dominance in contemporary aquatic environments indicates that they have developed unique and effective mechanisms to cope with the rapid and considerable fluctuations that characterize these environments. In view of their evolutionary history from a secondary endosymbiosis, inter-organellar regulation of biochemical activities may be of particular relevance. Diatom mitochondrial alternative oxidase (AOX) is believed to play a significant role in supplying chloroplasts with ATP produced in the mitochondria. Using the model diatom Phaeodactylum tricornutum we generated AOX knockdown lines, and followed sensitivity to stressors, photosynthesis and transcriptome and metabolome profiles of wild-type and knockdown lines. We show here that expression of the AOX gene is upregulated by various stresses including H2O2, heat, high light illumination, and iron or nitrogen limitation. AOX knockdown results in hypersensitivity to stress. Knockdown lines also show significantly reduced photosynthetic rates and their chloroplasts are more oxidized. Comparisons of transcriptome and metabolome profiles suggest a strong impact of AOX activity on gene expression, which is carried through to the level of the metabolome. Our data provide evidence for the involvement of mitochondrial AOX in processes central to the cell biology of diatoms, revealing that cross-talk between mitochondria and chloroplasts is crucial for maintaining sensitivity to changing environments.
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