期刊
NEW PHYTOLOGIST
卷 200, 期 2, 页码 511-522出版社
WILEY
DOI: 10.1111/nph.12386
关键词
ethylene; Ethylene Response Factor (ERF); Populus; tension wood; transcript analysis; wood development; xylem
资金
- FORMAS (FuncFiber/Bioimprove -centre of excellence in wood science)
- Vetenskapsradet
- Swedish Energy Agency
- EU
- VINNOVA
- Bio4Energy, the Swedish Programme for renewable energy
- TEKES
- Forest Cluster - Strategic Centre for Science, Technology and Innovation (FuncWood, EffTech and EffFibre)
- Office of Science of the US Department of Energy [DE-AC02-05CH11231]
Ethylene Response Factors (ERFs) are a large family of transcription factors that mediate responses to ethylene. Ethylene affects many aspects of wood development and is involved in tension wood formation. Thus ERFs could be key players connecting ethylene action to wood development. We identified 170 gene models encoding ERFs in the Populus trichocarpa genome. The transcriptional responses of ERF genes to ethylene treatments were determined in stem tissues of hybrid aspen (Populus tremulaxtremuloides) by qPCR. Selected ethylene-responsive ERFs were overexpressed in wood-forming tissues and characterized for growth and wood chemotypes by FT-IR. Fifty ERFs in Populus showed more than five-fold increased transcript accumulation in response to ethylene treatments. Twenty-six ERFs were selected for further analyses. A majority of these were induced during tension wood formation. Overexpression of ERFs 18, 21, 30, 85 and 139 in wood-forming tissues of hybrid aspen modified the wood chemotype. Moreover, overexpression of ERF139 caused a dwarf-phenotype with altered wood development, and overexpression of ERF18, 34 and 35 slightly increased stem diameter. We identified ethylene-induced ERFs that respond to tension wood formation, and modify wood formation when overexpressed. This provides support for their role in ethylene-mediated regulation of wood development.
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