期刊
NEW PHYTOLOGIST
卷 197, 期 4, 页码 1250-1261出版社
WILEY
DOI: 10.1111/nph.12091
关键词
defense-like reactions; insertion mutant; legume; nitrogen fixation; rhizobia; senescence; symbiosis; symbiosome
资金
- Centre National de La Recherche Scientifique (CNRS)
- Agence Nationale de la Recherche (ANR) Blanc International [SVSE 6.2010.1 (LEGUMICS)]
- French Ministry of Research
- National Science Foundation [DBI-0703285]
- Samuel Roberts Noble Foundation
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [1127155] Funding Source: National Science Foundation
Medicago truncatula and Sinorhizobium meliloti form a symbiotic association resulting in the formation of nitrogen-fixing nodules. Nodule cells contain large numbers of bacteroids which are differentiated, nitrogen-fixing forms of the symbiotic bacteria. In the nodules, symbiotic plant cells home and maintain hundreds of viable bacteria. In order to better understand the molecular mechanism sustaining the phenomenon, we searched for new plant genes required for effective symbiosis. We used a combination of forward and reverse genetics approaches to identify a gene required for nitrogen fixation, and we used cell and molecular biology to characterize the mutant phenotype and to gain an insight into gene function. The symbiotic gene DNF2 encodes a putative phosphatidylinositol phospholipase C-like protein. Nodules formed by the mutant contain a zone of infected cells reduced to a few cell layers. In this zone, bacteria do not differentiate properly into bacteroids. Furthermore, mutant nodules senesce rapidly and exhibit defense-like reactions. This atypical phenotype amongst Fix mutants unravels dnf2 as a new actor of bacteroid persistence inside symbiotic plant cells.
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