期刊
NEW PHYTOLOGIST
卷 195, 期 1, 页码 189-202出版社
WILEY
DOI: 10.1111/j.1469-8137.2012.04129.x
关键词
Colletotrichum higginsianum; defence; elicitor; NADP-malic enzyme (NADP-ME); pathogen-associated molecular pattern (PAMP); reactive oxygen species (ROS)
资金
- Deutsche Forschungsgemeinschaft (DFG) [MA2379/8-1, MA2379/8-2]
- [DFG-FOR666]
While photosynthetic NADP-malic enzyme (NADP-ME) has a prominent role in the C4 cycle, the biological function of nonphotosynthetic isoforms remains elusive. Here, we analysed the link between Arabidopsis thaliana cytosolic NADP-ME2 and the plant defence response. Arabidopsis thaliana plants with wild-type and modified NADP-ME2 expression levels were analysed after elicitation with pathogen-associated molecular patterns (PAMPs) and during the interaction with the hemibiotrophic fungal pathogen Colletotrichum higginsianum. Under normal growth conditions, the lack or gain of NADP-ME2 activity produced large changes in plant metabolite pool sizes without any effect on morphology or development. Total NADP-ME activity and NADP-ME2 transcript level were enhanced after PAMP treatment and pathogen infection. During infection with C.higginsianum, loss-of-function mutants of NADP-ME2 (nadp-me2) showed enhanced susceptibility. Transient apoplastic reactive oxygen species (ROS) production after elicitation and callose papilla formation after infection were dampened in nadp-me2. Late salicylic acid (SA)-dependent and SA-independent defence responses were not affected. Taken together, our results indicate that NADP-ME2 is an important player in plant basal defence, where it appears to be involved in the generation of ROS. Moreover, NADP-ME2 was found to be dispensable for later defence responses.
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