期刊
NEW PHYTOLOGIST
卷 193, 期 3, 页码 755-769出版社
WILEY
DOI: 10.1111/j.1469-8137.2011.03948.x
关键词
Glomeromycota; Glomus; meiosis and recombination genes; mycorrhiza; small secreted proteins; symbiosis; transcript profiling
资金
- Region Lorraine
- European Commission ENERGYPOPLAR [FP7-211917]
- Education Ministry of China
- Network of Excellence EVOLTREE [FP6-016322]
- US Department of Energy (DOE) - Oak Ridge National Laboratory Scientific Focus Area for Genomics Foundational Sciences
- German Research Foundation
- Regional Project Converging Technologies-BI-OBIT
- Spanish Ministry of Science and Education [AGL2009-08868]
- US National Science Foundation [IOS-0842720]
- DOE [DE-AC05-00OR22725]
- INRA
- Genoscope
- New Mexico University
- Michigan State University
- Ministry of Food of the FRG
- Ministry of Agriculture of the FRG
- Ministry of Consumer Protection of the FRG
- Brandenburg State
- Thuringen State
The arbuscular mycorrhizal symbiosis is arguably the most ecologically important eukaryotic symbiosis, yet it is poorly understood at the molecular level. To provide novel insights into the molecular basis of symbiosis-associated traits, we report the first genome-wide analysis of the transcriptome from Glomus intraradices DAOM 197198. We generated a set of 25 906 nonredundant virtual transcripts (NRVTs) transcribed in germinated spores, extraradical mycelium and symbiotic roots using Sanger and 454 sequencing. NRVTs were used to construct an oligoarray for investigating gene expression. We identified transcripts coding for the meiotic recombination machinery, as well as meiosis-specific proteins, suggesting that the lack of a known sexual cycle in G.intraradices is not a result of major deletions of genes essential for sexual reproduction and meiosis. Induced expression of genes encoding membrane transporters and small secreted proteins in intraradical mycelium, together with the lack of expression of hydrolytic enzymes acting on plant cell wall polysaccharides, are all features of G.intraradices that are shared with ectomycorrhizal symbionts and obligate biotrophic pathogens. Our results illuminate the genetic basis of symbiosis-related traits of the most ancient lineage of plant biotrophs, advancing future research on these agriculturally and ecologically important symbionts.
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