期刊
NEW PHYTOLOGIST
卷 187, 期 3, 页码 805-818出版社
WILEY
DOI: 10.1111/j.1469-8137.2010.03320.x
关键词
common bean (Phaseolus vulgaris); common bean microRNAs; manganese toxicity; nutrient-deficiency stress; nutrient-stress responsive microRNAs
资金
- Consejo Nacional de Ciencia y Tecnologia, Mexico (CONACyT) [083206, 200048]
- US Department of Agriculture [USDA-FAS MX161]
MicroRNAs (miRNAs) play a pivotal role in post-transcriptional regulation of gene expression in plants. Information on miRNAs in legumes is as yet scarce. This work investigates miRNAs in an agronomically important legume, common bean (Phaseolus vulgaris). A hybridization approach employing miRNA macroarrays -printed with oligo-nucleotides complementary to 68 known miRNAs -was used to detect miRNAs in the leaves, roots and nodules of control and nutrient-stressed (phosphorus, nitrogen, or iron deficiency; acidic pH; and manganese toxicity) common bean plants. Thirty-three miRNAs were expressed in control plants and another five were only expressed under stress conditions. The miRNA expression ratios (stress: control) were evaluated using principal component and hierarchical cluster analyses. A group of miRNAs responded to nearly all stresses in the three organs analyzed. Other miRNAs showed organ-specific responses. Most of the nodule-responsive miRNAs showed up-regulation. miRNA blot expression analysis confirmed the macroarray results. Novel miRNA target genes were proposed for common bean and the expression of selected targets was evaluated by quantitative reverse transcriptase-polymerase chain reaction. In addition to the detection of previously reported stress-responsive miRNAs, we discovered novel common bean stress-responsive miRNAs, for manganese toxicity. Our data provide a foundation for evaluating the individual roles of miRNAs in common bean.
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