期刊
NEW PHYTOLOGIST
卷 188, 期 3, 页码 762-773出版社
WILEY
DOI: 10.1111/j.1469-8137.2010.03422.x
关键词
Arabidopsis thaliana; mitogen-activated protein kinase 6; phosphatidic acid; phospholipase D; salt stress
资金
- NSF [MCB0455318, DBI 0521587, EPS-0236913]
- State of Kansas through Kansas Technology Enterprise Corporation
- Kansas State University
- Ministry of Science and Technology of China [2006CB100100, 2008AA10Z122]
- National Science Foundation of China [30625027, 90817014]
- Ministry of Education of China [B07030]
- Department of Education of Jiangsu Province [200910]
P>Phospholipase D (PLD) hydrolyzes phospholipids to produce phosphatidic acid (PA) and a head group, and is involved in the response to various environmental stresses, including salinity. Here, we determined the roles of PLD alpha and PA in the mediation of salt (NaCl)-stress signaling through the regulation of mitogen-activated protein kinase (MAPK or MPK) in Arabidopsis thaliana. NaCl-induced changes in the content and composition of PA were quantitatively profiled by electrospray ionization-tandem mass spectrometry (ESI-MS/MS). A specific PA species (a MAPK 16:0-18:2 PA), which was increased in abundance by exposure to NaCl, bound to a MPK6, according to filter binding and ELISA. The effect of PA on MPK6 activity was tested using in-gel analysis. 16:0-18:2 PA stimulated the activity of MPK6 immunoprecipitated from Arabidopsis leaf extracts. Treatment with NaCl induced a transient activation of MPK6 in wild-type plant, but the activation was abolished in the pld alpha 1 plant mutant. A plasma membrane Na+/H+ antiporter (SOS1) was identified as a downstream target of MPK6. MPK6 phosphorylated the C-terminal fragment of SOS1. The MPK6 phosphorylation of SOS1 was stimulated by treatment with NaCl, as well as directly by PA. These results suggest that PA plays a critical role in coupling MAPK cascades in response to salt stress.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据