期刊
NEW PHYTOLOGIST
卷 188, 期 3, 页码 726-739出版社
WILEY
DOI: 10.1111/j.1469-8137.2010.03409.x
关键词
Arabidopsis thaliana; cell elongation; cell wall; cellulose; Fourier transform infrared (FT-IR) microspectroscopy; hypocotyl; pectin de-methylesterification; transcriptome
资金
- French Ministry of Research and Technology [ACI BCMS195, ANR BLAN06-2_141830]
- EU [028974, 037704]
- University of Ghent
- Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT, Vlaanderen)
- Research Foundation, Flanders (FWO, Vlaanderen) [IUAP VI/33]
- University of Antwerp
- DFG [BI 1417/1-1]
P>We focused on a developmentally regulated growth acceleration in the dark-grown Arabidopsis hypocotyl to study the role of changes in cell wall metabolism in the control of cell elongation. To this end, precise transcriptome analysis on dissected dark-grown hypocotyls, Fourier transform infrared (FT-IR) microspectroscopy and kinematic analysis were used. Using a cellulose synthesis inhibitor, we showed that the growth acceleration marks a developmental transition during which growth becomes uncoupled from cellulose synthesis. We next investigated the cellular changes that take place during this transition. FT-IR microspectroscopy revealed significant changes in cell wall composition during, but not after, the growth acceleration. Transcriptome analysis suggested a role for cell wall remodeling, in particular pectin modification, in this growth acceleration. This was confirmed by the overexpression of a pectin methylesterase inhibitor, which caused a delay in the growth acceleration. This study shows that the acceleration of cell elongation marks a developmental transition in dark-grown hypocotyl cells and supports a role for pectin de-methylesterification in the timing of this event.
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