期刊
NEW PHYTOLOGIST
卷 188, 期 3, 页码 750-761出版社
WILEY
DOI: 10.1111/j.1469-8137.2010.03405.x
关键词
cell death; ENA ATPases; Na plus efflux; Physcomitrella patens; sodium antiporter; SOS1
资金
- Spanish Ministerio de Ciencia e Innovacion
- ERDF [AGL2007-61075]
- DGUI
- Biotechnology and Biological Sciences Research Council [BB/F001797/1] Funding Source: researchfish
- BBSRC [BB/F001797/1] Funding Source: UKRI
P>SOS1 is an Na+/H+ antiporter that plays a central role in Na+ tolerance in land plants. SOS1 mediation of Na+ efflux has been studied in plasma-membrane vesicles and deduced from the SOS1 suppression of the Na+ sensitivity of yeast mutants defective in Na+-efflux. However, SOS1-mediated Na+ efflux has not been characterized in either plant or yeast cells. Here, we use Physcomitrella patens to investigate the function of SOS1 in planta. In P. patens, a nonvascular plant in which the study of ion cellular fluxes is technically simple, the existence of two SOS1 genes suggests that the Na+ efflux remaining after the deletion of the ENA1 ATPase is mediated by a SOS1 system. Therefore, we cloned the P. patens SOS1 and SOS1B genes (PpSOS1 and PpSOS1B, respectively) and complementary DNAs, and constructed the Pp Delta sos1 and Pp Delta ena1/Pp Delta sos1 deletion lines by gene targeting. Comparison of wild-type, and Pp Delta sos1 and Pp Delta ena1/Pp Delta sos1 mutant lines revealed that PpSOS1 is crucial for Na+ efflux and that the Pp Delta sos1 line, and especially the Pp Delta ena1/Pp Delta sos1 lines, showed excessive Na+ accumulation and Na+-triggered cell death. The Pp Delta sos1 and Pp Delta ena1/Pp Delta sos1 lines showed impaired high-affinity K+ uptake. Our data support the hypothesis that PpSOS1 mediates cellular Na+ efflux and that PpSOS1 enhances K+ uptake by an indirect effect.
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