4.6 Article

Vibrational imaging based on stimulated Raman scattering microscopy

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NEW JOURNAL OF PHYSICS
卷 11, 期 -, 页码 -

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IOP PUBLISHING LTD
DOI: 10.1088/1367-2630/11/3/033026

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  1. Deutsche Forschungsgemeinschaft [VO 825/1-2, VO 825/1-3, VO 825/1-4]

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A stimulated Raman scattering microscope with near-infrared picosecond laser pulses at high repetition rates (76 MHz) and radio-frequency lock-in detection is accomplished. Based on stimulated Raman loss detection, we demonstrate noninvasive point-by-point vibrational mapping of chemical and biological samples with high sensitivity and without the requirement for labeling of the sample with natural or artificial fluorophores. We experimentally demonstrate a major benefit of this technique, which is the capability to respond exclusively to the linear Raman-resonance properties of the sample, thus allowing a direct quantitative interpretation of image contrast in terms of the number density of Raman-active modes.

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