期刊
NEW JOURNAL OF CHEMISTRY
卷 42, 期 17, 页码 14642-14647出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8nj02262c
关键词
-
资金
- National Natural Science Foundation of China [31471641, 31772068, 31701681]
- Special Project of Independent Innovation of Shandong Province [2014CGZH0703]
- Shandong Provincial Natural Science Foundation [ZR2014CM009, ZR2015CM016, ZR2016CM29, ZR2017BC001, ZR2014FL003]
- Key Research and Invention Program of Shandong Province [2017GNC10119]
- Key Innovative Project for 2017 Major Agriculture Application Technology of Shandong Province
A homogeneous type of electrochemical aptasensor was designed based upon the principle of target-induced and tool enzyme-assisted signal amplification, which was employed for the detection of profenofos residues. In this experiment, profenofos, a type of organophosphorus pesticide, was used as a model target. The electrochemical signals of the aptasensor were acquired via electrostatic adsorption of negatively charged screen-printed electrodes (SPEs) and positively charged ferrocene (Fc). The ingeniously designed hairpin structure of the probe was unfolded with profenofos bound to the aptamer sequences, and after this, the target profenofos and the complementary DNA sequences with hairpin probes (HP) were separately recycled with the help of Vent polymerase and T7 exonuclease (T7 Exo). At the same time, due to the employment of T7 Exo, the Fc labeled DNA duplex could be digested into mononucleotides, making the end-labeled Fc approach the electrode surface and generate an enhanced electrochemical response. Based on this strategy, an ultrasensitive electrochemical aptasensor was fabricated with a low detection limit (LOD) of 0.01 ng mL(-1). The developed method can be applied for the determination of profenofos residues in vegetables, and it produced satisfactory results.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据