期刊
NEW JOURNAL OF CHEMISTRY
卷 37, 期 11, 页码 3706-3715出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3nj00542a
关键词
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资金
- National Natural Science Foundation of China [20871056, 21171070, 21371075]
- Planned Item of Science and Technology of Guangdong Province [c1011220800060, c1211220800571]
- Natural Science Foundation of Guangdong Province
- Fundamental Research Funds for the Central Universities
Three ruthenium(II) complexes [Ru(bpy)(2)(biim)](2+) (1), [Ru(phen)(2)(biim)](2+) (2) and [Ru(p-mopip)(2)-(biim)](2+) (3) (where bpy is 2,2'-bipyridine, phen is 1,10-phenanthroline, biim is 2,2'-bisimidazole and p-mopip is 2-(4-methoxyphenyl)-imidazo-[4,5f]phenanthroline), have been synthesized and characterized. The interactions of human telomeric DNA oligomers 5'-G(3)(T(2)AG(3))(3)-3' (HTG21) with ruthenium(II) complexes were investigated via UV-vis, fluorescence resonance energy transfer (FRET) melting assay, polymerase chain reaction (PCR) stop assay, and circular dichroism (CD) measurements. The results indicated that the three ruthenium(II) complexes could stabilize the formation of human telomeric G-quadruplex DNA, and complex 2 was found to be the most efficient. In vitro cytotoxicity assay by MTT also showed that complex 2 was superior to complexes 1 and 3 in inhibiting the growth of cancer cells. Telomeric repeat amplification protocol (TRAP) showed that complexes 2 and 3 led to an inhibition of the telomerase activity, and complex 2 was the significantly better inhibitor. Flow cytometric analysis and evaluation of mitochondrial membrane potential demonstrated that complex 2 inhibited the growth of HeLa cells through induction of apoptotic cell death, as evidenced by the depletion of mitochondrial membrane potential in HeLa cells.
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