期刊
NEW JOURNAL OF CHEMISTRY
卷 32, 期 7, 页码 1228-1234出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/b801743c
关键词
-
A general method is described for the covalent attachment to carbon surfaces of sensitive biomolecules such as antibodies. First, N-hydroxysuccinimide-activated carbon surfaces are prepared by a step by step method involving an electrografting process and a monoprotected homobifunctional linker to ensure a good control of the surface modi. cation. Then, antibodies are introduced, at the last step of the modi. cation procedure, under native conditions at physiological pH, to minimize their denaturation. Comparison with a classical method involving a homobifunctional linker shows a decrease of the degree of coverage of the surface by antibodies, explained by the formation of bridged structures by the linker.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据